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into the lentiviral vector system and investigate its abil-ity to deliver and regulate transgene expression in vitro and in vivo. We report that the entire Tet system can be incorpo-rated into a single lentiviral vector. This novel, inducible lentiviral vector can be produced in high titers and whenSri vidya sadhana
- For stable loss-of-function experiments, you should generate lentiviral particles and infect the target cells. Addition of puromycin will allow you to select for cells that stably express your shRNA of interest. The second generation system has one packaging plasmid which includes all the important packaging components: Gag, Pol, Rev, and Tat.
- For stable loss-of-function experiments, you should generate lentiviral particles and infect the target cells. Addition of puromycin will allow you to select for cells that stably express your shRNA of interest. The second generation system has one packaging plasmid which includes all the important packaging components: Gag, Pol, Rev, and Tat.
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